Monday, October 5, 2015

Egg Cell Macro Molecules Lab

In this lab, we asked the question of "Can macro-molecules be identified in an egg cell?". I found that egg membrane, egg yolk, and egg white had polysaccharides, proteins, and lipids. I thought that egg yolk would have lipids and monosaccharides, egg membrane would have proteins and polysaccarides, and egg white would have protein and polysaccharides. But the results were different. For monosaccharides, only the egg yolk and egg white had monosaccharides, the egg membrane didn't. We knew that the egg white and egg yolk both had monosaccharides when we added Benedicts solution, and they both turned dark and light green. We knew that the egg membrane didn't have monosaccharides because after adding Benedicts solution, the egg membrane turned light blue. We knew that egg membrane, egg yolk, and egg white all had polysaccharides because they all turned shades of black when we added iodine. When we added sodium hydroxide, the egg membrane. yolk, and white all turned purple. The egg yolk turned dark purple, and the egg membrane was light purple. Sudan III turned egg membrane and egg white into a shade of pink and orange. It turned egg yolk completely orange, proving that there were lipids inside them. The result was likely caused by the fact that monosaccharides, polysaccharides, proteins, and lipids, were inside the egg membrane, egg yolk, and egg white.
Our data was unexpected because we could have had bits of egg white with egg yolk, and egg white with the egg membrane. This could have made the indicators change color because there was some different part of the egg part, making it think the macromolecule was present. Due to these errors, in future experiments I would recommend gently putting the cell membrane into water before testing, and letting all of the egg white out before putting the egg yolk down, making the chance of egg white interfering less. Also, our data was unexpected from the recording of color. If a color was very dark brown, someone might write black, and dark pink might be red. In future experiments, I would recommend having a reference sheet of colors to look at, to match the closest color.
This lab was done to demonstrate how different parts of an egg are like a cell. From this lab, I learned what macromolecules are in different parts of the egg, which helps me understand the concept of a cell. Based on my experience from this lab, I could know what to eat for each macromolecule.

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